Bacteriological and molecular identification Of Pseudomonas Isolat-ed From Sheep In Baqubah Province

Abstract

Background: Pseudomonas cause a wide range of infections in animals.
Aims: Detection of P. aeruginosa from wounds cases of sheep by culture tech-nique and PCR
Methods :forty wound swabs from sheep were collected and cultured on blood and MacConkey, pseudomonas agar . PASS primers used for (PCR) based iden-tification
Results :
A total of 3/40 (7.5%), wound swabs were identified as P. aeruginosa after full morphological and biochemical tests and by PCR using the PASS primers for species level . The results pointed out that all 3 selected isolates gave positive re-sults, and the gene size was ≈ 950 bp.
Conclusion: the PCR approach aids the rapid detection of pseudomonas to re-duce financial loss. remarkable, and they were fast and accurate.