Bacteriological and molecular identification Of Pseudomonas Isolat-ed From Sheep In Baqubah Province

Authors

  • Shaymaa Jabbar Hassoon
  • Osama Ghazi Jalil
  • Hiba Ibrahim Ali
  • Wasan Saher Hassan
  • Eqbal Salman Najem

DOI:

https://doi.org/10.71375/djvs.2023.01402

Keywords:

Pseudomonas, Molecular diagnosis, Polymerase chain reaction

Abstract

Background: Pseudomonas cause a wide range of infections in animals.
Aims: Detection of P. aeruginosa from wounds cases of sheep by culture tech-nique and PCR
Methods :forty wound swabs from sheep were collected and cultured on blood and MacConkey, pseudomonas agar . PASS primers used for (PCR) based iden-tification
Results :
A total of 3/40 (7.5%), wound swabs were identified as P. aeruginosa after full morphological and biochemical tests and by PCR using the PASS primers for species level . The results pointed out that all 3 selected isolates gave positive re-sults, and the gene size was ≈ 950 bp.
Conclusion: the PCR approach aids the rapid detection of pseudomonas to re-duce financial loss. remarkable, and they were fast and accurate. 

 

Downloads

Download data is not yet available.

Downloads

Published

01-12-2023

How to Cite

Hassoon, S. J. ., Jalil, O. G., Ali, H. I. ., Hassan, W. S. ., & Najem, E. S. . (2023). Bacteriological and molecular identification Of Pseudomonas Isolat-ed From Sheep In Baqubah Province. Diyala Journal for Veterinary Sciences, 1(4), 15–28. https://doi.org/10.71375/djvs.2023.01402

Issue

Vol. 1 No. 4 (2023): Diyala journal for Veterinary Sciences

Section

Articles

License

Copyright (c) 2023 Shaymaa Jabbar Hassoon, Osama Ghazi Jalil, Hiba Ibrahim Ali, Wasan Saher Hassan, Eqbal Salman Najem

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.