Isolation and identification of Pathogenic Bacteria from Cyprinus carpio L. by Vitek 2 System

Abstract

Fish are vulnerable to a wide range of bacterial infections that are naturally occurring in the surroundings. The current study's goal was to isolate the pathogenic bacteria from common carp sold in ponds at the city of Baqubah which utilize as most popular fish mail and principle food. Sixty fish samples were obtained from five places in Baqubah city (12 samples from AL-Mafraq, 12 samples from AL-Razi, 12 samples from AL-Moalemmen District , 12 samples from AL-Rahma and 12 samples from Old Baqubah). The samples were taken from fish from each location. Anatomical process to the each fish was done and then bacterial strains were isolated from several parts of fish body (skin, gills ,liver and intestine ). All samples were cultivated on blood agar medium as an enriched medium and MacConkey agar as a differentiated medium after collection, and they were both hatched on 37 ° C for 24 hr. The colony morphology, microscopic analysis, and biochemical assays were used to identify probable isolates. , then confirmed and sensitivity were done by VITEK 2 system. The bacterial isolated from each target organs showed that the potential pathogenic bacteria which establish in carp from this investigation were E.coli 30%,Klebseilla spp 30% ,Pseudomonas spp 30% and salmonella spp 10% in addition to other species such as Staphylococcus spp, Proteus spp and others. Sensitivity test that performed by Vetk2 system showed high rates of resistance to the wide spectrum antibiotics which commercially used in Iraq. Fish handling or preparation errors may put consumers' food safety at risk because of the existence of pathogenic bacteria in the fishes.