Histopathological Changes of Serratia Marcescens In Mice

Abstract

A prominent opportunistic pathogen that affects a range of hosts, including
invertebrates, plants, and mammals, has been identified as the cause of Serratia
marcescens, a gram-negative bacillus belonging to the Enterobacteriaceae family.
Numerous clinical conditions are associated with it, such as meningitis, pneumonia,
keratitis, urinary tract infections, and wound infections; Given that its red colonies are
easily identifiable, Serratia marcescens is utilized as a biological marker. The current
research sought to investigate the alterations in mice immunized against Serratia
marcescens infection with (WCSA-S) of S. marcescens and (KWCA-S) of S. marcescens.
Twelve male albino mice were split up randomly into three groups (4 mice for each group)
and their histopathological changes examination was performed. The first group was
vaccinated with WCSAg-S (500 μg/ml) vaccine subcutaneously (S/C). The third group (the
negative control) was given PBS (1 ml) subcutaneously, while the second group received
KWCA-S (9 x 108cfu/ml) vaccinations (pH 7.2). After 14 days, the male albino mice were
given booster doses of the same antigens.. after 28 days of immunized groups with infective
dose (1x106 cfu/ml) of Serratia marcescens in order to determine the histopathological
changes in the internal organs (liver, spleen, kidneys, lungs, and intestine) that showed
severe histopathological changes in the positive control groups compared with other
immunized groups.