Molecular detection of MecA , Blaz Genes and phenotypic detection of Antibiotic Sensitivity Pattern For S.aureus And MRSA Isolated From Dermal lesions of Sheep In Diyala Governorate - Iraq

Abstract

Back ground: S. aureus is one of the dominant bacterial pathogens among dermal infections in human and animals, which have resistance to different antimicrobial drugs.
Objectives : Isolation and identification of S.aureus from skin lesions among sheep by traditional methods, Vitek 2 system and PCR using Staur 4, 6 specific primers for validation, Detection of genes (methicillin resistant (mecA), Beta- lactamase gene (blaZ) by conventional PCR ,determine antibiotic sensitivity pattern by kirby bauer disc diffusion method.
Methods:
A total of 75 swaps were collected from sheep suffered from variety of infected skin lesions (wounds, abscesses, dermatitis, abrasion) recording to detect methicillin sensitive and resistant s. aureusby employing traditional methods in addition to confirmatory techniques through fast rap- id VETEK2 system and PCR and ,determine antibiotic sensitivity pattern by kirby bauer disc diffusion method.
Results
S. aureus was isolated from 46/75 ,61.33% of sheep samples .MRSA was recovered from 14 / 46,( 30.43%), which represent 14/75 , 18.67%) of total sheep samples with positive mecA gene using PCR. All sheep S.aureus isolates have positive detection for Staur 4, 6 primers (100%), blaZ (100%).
Levofloxacin and Ofloxacin resistance was reported in 16/46,(34.78%). A total of 14/46,(30%) of S.aureus have resistance for methicillin that was confirmed early by detection of MecA gene . Oxacillin and erythromycin resistance was reported in 8/46, (17.39%). Vancomycin resistance was reported in 4/46, (8.69%). Absolute sensitivity was reported for ciprofloxacin ,gentamycin, tetracycline, rifampicin, imipenem and chloramphenicol.

Non Multidrug resistant S.aureus isolates have resistant for fluoroquinolones 8/46,( 17.39%), Penicillins 6/46(13.04%), polypeptide antibiotics, 4/46,(8.69%). Multidrug resistant S.aureus isolates have resistance for Penicillins, Fluoroquinolones, Macrolide ,8/46,(17.39%).
Conclusions :Methicillin sensitive S.aureus was more common compared with MRSA isolated from dermal infections of sheep. Blaz gene was predominantly expressed by S.aureus isolates followed by Mec A gene. Levofloxacin and Ofloxacin resistance were higher followed by Oxa-cillin . erythromycin and vancomycin. Multi drug resistant trait commonly for Penicillins, Fluo-roquinolones, Macrolide