The Alteration of Blood Picture and Histopathological Changes by Proteus Mirabilis in Albino Male Rats

Abstract

Thirty (30) male rats used in the study and were divided equally into three groups: 1^st  group (GI): given (1CC /animal) an oral dose of phosphate buffer saline (PBS) by a stomach tube as a control group for 60 days, 2^nd  group (GII): One dose weekly were given 1CC viable Proteus Mirabilis (1 X 10⁹ CFU/ml) orally by stomach tube for 60 days, 3^rd group (GIII): twice dose weekly were given 1CC viable Proteus Mirabilis (1 X 10⁹ CFU/ml) orally by stomach tube for 60 days. After 60 days of experiment collected the blood from heart for hematological analysis and collected tissue sample from kidney and urinary bladder were taken for pathological examination.

The results of hematological analysis showed significant decrease in RBC, Hb, HCT, platelets, while significant increase in WBC, neutrophils, lymphocytes & monocytes in 2^ed group and 3^ed group compare with control group.

The histopathological changes of Kidney in 2^nd group include: all the cell lining of the proximal convoluted tubules are swollen due to acute cellular swelling and mild infiltration of inflammatory cells, other section showed bands of interstitial fibrosis with dense infiltration of mononuclear cells, Dense protein cast in the tubules, In 3^rd group showed all tubules hydropic ( acute cellular swelling ), heavy infiltration around glomeruli and in the interstitial with chronic inflammatory cells, most of one lobule is necrotic and disrupted with many nuclear fragment and adherent to epithelial of Bowman capsule.

The histopathological changes of urinary bladder in 2^nd group showed thickened connective tissue of stroma, ulcer of transitional layer, edema with mononuclear cells infiltration, also congested of blood vessels inside the gland, congested blood vessels in the muscular layer with monocytes, In 3^rd group showed chronic inflammatory cells infiltration mostly macrophage in the lumen and stroma of bladder, congested of blood vessels in submucosal layer, homogenous to hyalinized like mucosa, squamous cells of transitional layer.

The aim and position of this study to clarify the belongings of Proteus Mirabilis on blood profile and kidney with urinary bladder tissue after exposure orally.