Study the Effects of Turmeric Extract Nanoparticles Gel on the Healing of Achilles Tendon Injury in Rats

Abstract

This study investigates the healing effects of Turmeric extract nanoparticles gel extracted from Turmeric root on Achilles tendon injury in Wistar rats, comparing its efficacy with control group that received no treatment. A total of 20 Wistar rats were randomly divided into two groups, with each group consisting of 10 animals. The first group was control group that dropped with 0.2 ml of phosphate buffer saline (PBS) and the second group treated with (0.44mg /kg) 0.13 ml of turmeric extract nanoparticles gel. The treatment was applied topically once after making tendon injury induced by exposing the tendon to cutting and anastomosis. The clinical evaluations were done daily until 28 days post operation. Histopathological examination was achieved on 14 days as well as 28 days post operation to evaluate healing process. The animals were housed in metal-topped plastic cages under standard laboratory conditions, with unrestricted access to food and water. The histopathological observations showed the presence of thick and regular arranged collagen fibers in the Turmeric-treated group (G2), numerous dilated and engorged blood vessels are evident, as well as new blood vessel formation at site of injury, these effects promote collagen production and tenogenesis. Animals’ appetite and movement levels rapidly returned to normal. The results of clinical evaluation at 28 days post operation displayed that all variables of the injured tendons were significantly differed (P< 0.05) from the control group. In conclusion, the current study demonstrated that the Turmeric extract group accelerated and improved healing of the injured Achilles tendon based on clinical and histopathological findings. These findings corroborated the indicators of improvement as well as the Turmeric group was the superior due to the synergistic effects obtained on advantage in quality and quantity of healing tendon formatting in a period of 28 days postoperatively, and this demonstrated both mechanical and histological support to the regeneration of the injured tendon. The study aimed to the persistent challenges in improving tendon recovery and restoring its normal function, this study aimed to: Evaluating the efficacy of Turmeric Extract Nanoparticles gel on healing and regeneration of Achilles Tendon injury through: 1-Clinical evaluation. 2-Histopathological examination.