Isolation and Identification of Pseudomonas aeruginosa from Bovine Urine in Diyala Governorate

Abstract

Pseudomonas aeruginosa is a Gram-negative, aerobic rod from the Gamma-proteobacteria, widespread in humans and the environment. Its genetic diversity, adaptability, antibiotic resistance, and biofilm formation make it a clinically important pathogen causing urinary, wound, respiratory, and gastrointestinal infections. This study aimed to isolate and characterize Pseudomonas aeruginosa from cow urine samples collected between September 1, 2024, and May 1, 2025, in different regions of Diyala Governorate. Isolation was performed using cetrimide agar and MacConkey agar, and the isolates were confirmed by the VITEK 2 system. A total of 93urine samples were collected from different regions of Diyala Governorates, 85 (91.4%) showed positive growth, while 8 samples (8.6%) showed no growth. Among all the positive samples, Pseudomonas aeruginosa was identified in 7 isolates (8.23%) through morphological and biochemical tests, and confirmation was achieved using VITEK 2. In conclusion, urine samples from cows are a common source of Pseudomonas aeruginosa, and the VITEK2 system is reliable for identifying the bacteria.