Gliotoxin production from Aspergillus fumigatus isolated from Bovine Respiratory Infection

Abstract

Background: The gliotoxin-producing capability of A. fumigatus isolates through High-Performance Liquid Chromatography (HPLC) determination. The results of this research revealed that among the 30 samples, A. fumigatus showed the highest yield of gliotoxin when cultivated on Yeast Extract Sucrose (YES) medium with a gliotoxin content of 23.823 μg /kg as determined by HPLC. The primary goal of the study was to synthesize gliotoxin (GT) by Aspergillus fumigatus. The research resulted was conducted in the period between February 2022 and March 2023. Fifty nasal swabs were taken from animals in the Baghdad province with respiratory tract infection. All the samples received were grown on Czapek Dextrose Agar (CDA). The percentages of fungi isolated from nasal samples were as follows A. fumigatus (60%), A. niger (10%), Aspergillus spp. (16%), Rhizopus spp. (4%), Mucor spp. (2%), and Candida spp. (4%). To establish the identification of each isolate, both the macroscopic and the microscopic examinations were used. Aims: The primary goal of the study was to synthesize gliotoxin (GT) by Aspergillus fumigatus. Results: Samples, which were collected from the nasal cavities of suspected animals having clinical symptoms from different areas in Baghdad Province, were cultured on czapek dox agar. The culture and microscope examination revealed 30 isolates for the infections were positive and identified as Aspergillus fumigates, whereas 20 isolates corresponded to other fungal species. The findings of this investigation revealed that Yeast Extract Broth (YEB) supplemented with 4% sucrose and 2% yeast extract (YES) and incubated at 37 °C for seven days served as an efficient