Early Induction of Estrous Cycle Experimentally in Laboratory Mice

Abstract

Background: The early induction of heat in laboratory animals like mice is so essential for reproductive performance, and detection of estrus could be achieved by many ways like visual evaluation, vaginal cytology. Mice could be artificially induced to heat period to get them receptive for male.

Aims: To induce estrous cycle in mice early than normal puberty

15 young mice age 4 weeks grouped to three groups, first group treated as control, second group received 0.05 ug estradiol benzoate while third group treated with 0.05 ug GnRH s/c., the same dose for two groups 2 and 3 repeated after three days. Vaginal cytology smears were taken 48 hours after last injection for animals of this study. The results of the study revealed significant showing of estrus behavior in group 2 compared with other groups that used hormones accelerate signs of estrus in mice in early stages.

Conclusion: Setting of the scope and period of the heat cycle and the creating of the heat are helpful in the learing, examine and assessment the impacts of hormone on the reproduction performance.