Efficiency of PMSG +HCG hormones in improvement pregnancy rate and reproductive performance in anestrus domestic cats (Felis catus)

Abstract

Abstract
This study was designed to investigate the role of PMSG+ HCG hormone to improve ovulation in cats after natural breeding during out-of season and their effect to some reproductive parameters in domestic queen. Twelve domesticated cats of known origin, age between 1-3 years old, addition to three a fertile male, cats were divided randomly into three equal groups; the first group of 4 queans was control (negative control) , the second group 4 queens treated with 200 IU PMSG hormone (positive control) to induction estrus cycle because the cats are were out of the breeding season, the third group 4 queens treated with 200 IU PMSG and 150 IU HCG. All females’ three groups are were released with males during estrus to natural breeding. Blood collecting from jugular vein (2ml) before treatment in day zero and after treatment in day 14 to study the effect of treatment on level of progesterone hormone value. The male copulation and making a swab from the vagina to confirm estrus diagnosis. Also, in days 25 ultrasound techniques used to detect pregnancy then the animals follow up until parturition to detect gestation period, number of kitten birth and viability of kitten.
The result of the current study records the control group not treated with PMSG did not show signs of estrus during the treatment period, while the two group when injected with PMSG showed estrus except one cat in one of the groups did not show estrus. The result of duration of response shows high significant effect in control group in p ≤ 0.05 and showed the signs of estrus after 27± 7.00 days in two queens than treatment group (4.50± 0.86 and 3.67 ±0.88) in (PMSG and PMSG+HCG) groups respectively, also non-significant effect between two treatment groups in duration response. Also, theduration estrus (estrus phase) was 4.5 - 7 days did not have a significant difference between the two-treatment group (7.00±0.5 and 6.33±1.68) in (PMSG and HCG + PMSG) respectively than control group (4.50 ±.050). The result of present study we show the lowest pregnancy rate was in the control group and the PMSG group, followed by the HCG group high value increasingly. Also, the percentage of pregnancy rate high mathematically in PMSG +HCG group (66.7 %) than control, PMSG groups when the pregnancy rate percent was (50 %, 50%) respectively. The results of the current study show no significant effect in the pregnancy period, litter size, and viability rate in control group than two treatment group. Regarding the effect of treatment with HCG hormone to induced ovulation to progesterone hormonal value, we show increased the progesterone hormone after days 14 because induced ovulation and increased the number corpus luteal after used this program. Conclusion used of HCG to induce ovulation in domestic queens has no significant effect when the male is present with the females throughout the estrous phase but it lead to increase the pregnancy rate and progesterone value mathematically.