Isolation Escherichia coli from Clinical Mastitis of Dairy Cattle in Diyala Governorate

Abstract

Background: Mastitis is worldwide distributed disease affect farm animals especially cattle and cause economic losses characterized by decrease of milk production. This study aimed to determine the percentage of  Ecoli infection of dairy cattle in diyala governorate. Method: Isolation of E. coli by using MacConkey and EMB agers then confirm the isolates by PCR method depending on the 16s ribosomal gene . Result: The result represent that there were 20 samples of clinical mastitis milk give positive result about 40  of the total samples that collected, while there were 30 samples of clinical mastitis give negative result about 60  of total collected samples. The E.coli isolates were give positive result for 16s ribosomal gene. Conclusion: E. coli was common cause of mastitis in the cattle with high prevalence.  

Key words: E. coli, mastitis, cattle