The Activity of Eucalyptus Leaves Extract against some Bacteria isolated from Cattle Minced Meat in Diyala Province

Abstract

The Meat and Food Manufacturing are susceptible to contamination and many other foodborne diseases that can arise in food manufacturing areas due to poor personal hygiene and sanitation practices, leading to the growing of multiple pathogenic agents. Beef can be a foodborne pathogens due to unsanitary conditions during animal slaughter and meat processing. In recent decades, scientific strategies have been developed to reduce meat contamination and its effects on human health.
Forty meat samples were randomly collected from several butcher shops in Diyala province, minced, and prepared for the experiment to know the antibacterial activity of Eucalyptus leaves. Leaves were collected from the garden of Diyala University, cleaned and ground. Eucalyptus leaves extract was prepared against some bacteria isolated from minced meat. The aqueous extract of eucalyptus leaves was prepared at different concentrations (100%, 85%, 70%, 55%, and 40%) and used as an antibacterial against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Klebsiella spp.) investigated the antibacterial activity of Eucalyptus leaves extract toward some bacteria isolated from minced meat, Forty meat samples collected randomly from Many meat shops Diyala province, water extract ( 100%, 85%, 70%, 55% and 40%) prepared from Eucalyptus leaves and used as antibacterial activity against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Klebsiella spp.), Eucalyptus leaves were collected from the garden of Diyala University, the leaves was cleaned and ground. 25 grams of Eucalyptus leaves were put in 150 ml deionized water, placed on a hot stirrer plate at 90°C for 1 hour, and left for 5 hours, then purified using Whatman No. 1 filter paper. Isolated bacteria were identified based on colony description, gram stain, coagulase test, and catalase test—the antimicrobial activity of the extract against isolates carried out by the good diffusion method.
Results of this study revealed the prevalence of contamination of meat with Staphylococcus aureus spp and Klebsiella spp (65, 52.5) % respectively. Eucalyptus leaves extract displayed great activity against Staphylococcus aureus and Klebsiella spp. The highest antibacterialactivity was (33, 20 mm) against Staphylococcus aureus and Klebsiella spp. respectively, while the lowest activity against these bacteria was (21, 10) mm respectively.