Isolation and Identification of Klebsiella pneumoniae causing Urinary Tract Infection and Histopathological Changes of the Bladder and Kidney in Slaughtered Cattle

Abstract

Aims:
Isolation and identification of Klebsiella pneumoniae from urine of breeders and cattle , relationship of infection rate with age and gender and histopathological changes in bladder and kidney of slaughtered cattle.
Methods: One hundred urine samples were collected from breeders and cattle raised in villages in Diyala province (50 from breeders, and 50 from cattle) from different ages, genders, and areas. The samples of urine were examined by microscopic examination ,cultured on MacConky and eosin methylene blue agar ,and subjected to full biochemical identification by vitek 2 system and histopathological study for bladder and kidney in slaughtered cattle.
Results:
Before collection of urine sample the main clinical signs showed in some affected cows with urinary tract infection (UTI) represented by weight loss and an abrupt reduction in feed intake and milk production specially dairy cow depression, weakness and frequent urine dribbling. Whenever in breeders represented clinically by cloudy urine, fever, strong unpleasant smell of urine, dark or bloody urine, flank or back pain, burning with urination. A total of 28/50 (56%) of urine specimens give positive urine culture among breeders, 4(8%) were klebsiella pneumoniae. A total of 44/50(88%) of urine specimens give positive urine culture among cattle,4(8%) were klebsiella pneumoniae. Klebsiella Pneumoniae appear pink mucoid colonies on macConky agar. Histopathological sections of kidneys shows granuloma formation (macrophages and lymphocytes) and damage in tubules, there is severe damage in glomeruli, tubules with hemorrhage.Urinary bladder shown thickening of mucosa with sloughing and infiltration of inflammatory cells with edema .