Serological and Molecular Detection of Infectious laryngotraceitis (ILT) Outbreak in Layer flocks in Diyala Province

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Anmar Ayoub Al-Obiadi
Karim Sadun Al-Ajeeli

Abstract

A respiratory infection ranging from severe to mild was affecting 15 layer flocks of varying ages (70, 120, and 180 days old) at five distinct chicken farms located in five different districts within the province of Diyala. Clinical symptoms and postmortem findings were typical of infectious laryngotracheitis disease (ILTD) and ranged from mild or moderate to severe strains of ILTV infections, depending on the severity of the illness. In this detection, ELISA was performed using a customized commercial kit, and RT-PCR was performed using two sets of primers specific to the gG gene of the ILTV field strain and gG –ve for the attenuated vaccination strain. High titers of IgG antibodies to ILTVwere detected by using of ELISA test in all flocks ranged from 523.78 ± 84.938 to 1018.83 ± 83.965 units for layer flocks of 70 days old, and from 767.39 ± 137.502 to 2439.83 ± 275.142 units to 120 days old layers, and from 1832.61 ± 305.582 to 6842.50 ± 567.192 units for layer flocks of 180 days old. In RT-PCR test, all collected samples from tracheal and Laryngeal tissues of infected layers in five districts were positive for field strains and negative for vaccine strain. Their cycle thresholds (Ct) ranged 19.5 for the detected virus from layer flocks of Al-Khales district to 28.9 for the detected virus from layer flocks of Kanaan district.The detected virus is a circulating field strain and not vaccinal attenuated virus strain.

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How to Cite
Al-Obiadi, A. A. ., & Al-Ajeeli, K. S. . (2022). Serological and Molecular Detection of Infectious laryngotraceitis (ILT) Outbreak in Layer flocks in Diyala Province. Diyala Journal for Veterinary Sciences - Print ISSN: 2410-8863, 1(5), 96–112. Retrieved from https://djvs.uodiyala.edu.iq/index.php/djvs/article/view/62
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