Probiotic potential and Antimicrobial Properties of Lactobacillus plantarum isolated from Cow's Milk against Bacterial Pathogens isolated from Burn Infection
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This study aimed to assess antimicrobial potential of cell free supernatants (CFS) of Lactobacillus plantarum, alone, and in combinations with the commonly prescribed antibiotics against the isolated bacteria from burn infections. In this study, 42 samples of cow's milk were collected from different areas in Baghdad. Lactobacillus plantarum coded as spp.1, spp.2, and spp.3 were isolated and identified from cow's milk. Moreover, 187 burn swabs were collected from patients with burns infections attended to Burns Specialized Hospital/Medical city in Baghdad. The manual biochemical test and VITEK 2 system were used to identified L. plantarum species and bacterial pathogens causing burns infection. Of the total, Pseudomonas aeruginosa (34.22%) and Staphylococcus aureus (27.27%) were the most predominant isolates, and were multi-drugs resistant species. When cell free supernatants was added to antibiotic discs, the zone of bacterial growth inhibition were increased in some of these antibiotics. The highest auto-aggregation and co-aggregation percentages were reported after 24 hrs of incubation, 87.2% for L. planterum spp 2 and 88.4% for L. planterum spp.2 and P. aeruginosa. Co-aggregation of Lactobacilli indicated their ability to compete with the pathogens on nutrients and adhesion sites. Furthermore, CFS of L. plantarum found to enhance the antimicrobial activity of certain antibiotics.